1 Discipline of Biochemistry, Faculty of Medicine and Pharmacy, University of Oradea, Oradea, Romania Romania
2 Discipline of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania
3 Discipline of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania
Solidago graminifolia L. Salisb. is one of the latest species appeared in Romania. Due to the interest for the Solidago species as medicinal plants, we researched its phytochemical composition in comparison with the other three species present in Romania: Solidago virgaurea L., Solidago canadensis L., Solidago gigantea Aiton. Starting from the chemotaxonomic value of flavonoids, and their valuable biological properties, we wanted to analyze these substances from S. graminifolia compared to other Solidago species in Romanian flora. The studied species have a high content of flavonoids (3.44-5.21%). The flavonoid substances identified in the indigenous species of Solidago have a high chemotaxonomic value, each species having a charateristic chromatographic profile, therefore their analysis is useful in the case of adulterations. The qualitative analysis of flavonoids was performed by TLC and HPLC-MS, mean while the quantitative determination was achieved by spectrophotometric method and individual fractions separated by HPLC-MS.