Mammalian hormones are shown to affect not only regulation of mammalian homeostasis but also play roles in cross-talk between microorganisms and their hosts. In our study, the roles of norepinephrine (NE) at two different concentrations on growth alterations, adhesion – invasion of Pseudomonas aeruginosa were investigated.
The effects of NE on the growth, adhesion – invasion of P. aeruginosa ATTC 27853 were examined in human lung carcinoma (A549) cell culture. We analysed two concentrations (HNE: 0.04μg/mL and LNE: 0.0017 μg/mL) of NE considering NE’s psychological levels in a healthy individual to imitate in vivo conditions of the host. Bacterial counts of growth and adhesion-invasion were examined by the colony counting method. Growth of bacterium was significantly reduced in the presence of NE at 90’ incubation (p: 0.0004 for high concentration and p: 0.0003 for low concentration); on the other hand, at 180’ minutes incubation, only low concentration reduced the growth (p˂0.0001). While adhesion was increased in the presence of low NE concentration (p: 0.013), there was no alteration in invasion of P. aeruginosa in the presence of NE (p˃0.05). Our results suggested that NE has different effects on the growth and adhesion of P. aeruginosa and these effects depend on concentration and incubation period.
Fatma Kalaycı-Yüksek 1*, Defne Gümüş 1, Varol Güler 2, Mine Anğ-Küçüker 1
1 Istanbul Yeni Yüzyıl University, Faculty of Medicine, Department of Medical Microbiology
2 Istanbul Yeni Yüzyıl University, Faculty of Medicine, Department of Medical Biology and Genetics, Istanbul, Turkey
1 Istanbul Yeni Yüzyıl University, Faculty of Medicine, Department of Medical Microbiology
2 Istanbul Yeni Yüzyıl University, Faculty of Medicine, Department of Medical Biology and Genetics, Istanbul, Turkey
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